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Plos One : Alternative Sigma Factor Over-expression Enables Heterologous Expression of a Type II Polyketide Biosynthetic Pathway in Escherichia Coli, Volume 8

By Appanna, Vasu, D.

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Book Id: WPLBN0003943629
Format Type: PDF eBook :
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Reproduction Date: 2015

Title: Plos One : Alternative Sigma Factor Over-expression Enables Heterologous Expression of a Type II Polyketide Biosynthetic Pathway in Escherichia Coli, Volume 8  
Author: Appanna, Vasu, D.
Volume: Volume 8
Language: English
Subject: Journals, Science, Medical Science
Collections: Periodicals: Journal and Magazine Collection (Contemporary)
Historic
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Publisher: Plos

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Appanna, V. D. (n.d.). Plos One : Alternative Sigma Factor Over-expression Enables Heterologous Expression of a Type II Polyketide Biosynthetic Pathway in Escherichia Coli, Volume 8. Retrieved from http://worldebookfair.org/


Description
Description : We have demonstrated a new mechanism for heterologous expression of the oxytetracycline polyketide biosynthetic pathway, where high-level pleiotropic sigma factors from the heterologous host directly and positively regulate transcription of the non-native biosynthetic gene cluster. Our bioinformatics analysis is consistent with the hypothesis that heterologous expression mediated by the alternative sigma factor s54 may be a viable method for the production of additional polyketide products.

 

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